Mouse | CellWiki

Mouse

Charles River

Conell (2015)

Salafranca (2023)

Additional information

This page describes the morphology of an animal and is particularly intended for an insight into another world. The findings are based on only a few observations from residual material from animal experiments and have been assessed from a human diagnostic perspective. The differentiation algorithms of the human Sysmex XN equipment are not suitable for animal material. Students or professionals in veterinary medicine are advised to consult other sources of information for diagnostic purposes.

Conell (2015)

Mestas (2004)

The mouse blood smear combines features that would be appropriate in humans for both regeneration and myeloproliferation, with reticulocytosis, pronounced thrombocytosis and a strong lymphocytic differential, complemented by subtly abnormal neutrophils and fragile lymphocytes.

XN Scatterplots

wdf

wnr

pltf

ret

SFL

SSC

Lymphocytes

Monocytes

Neutrophils

Eosinophils

Basophils

Leukocytes

Basophils

NRBCs

Debris

Erythrocytes

Reticulocytes

RBC fragments

Platelets

immature platelets

Lymphocytes

Monocytes

Granulocytes

Reference

Focus

In the WDF channel, it can be seen that the neutrophils are almost completely huddled up to the lymphocyte population. The eosinophilic granulocytes are also shifted to the left, causing them to be wrongly classifying as neutrophils . In the PLT-F channel, a very large and broad platelet population is visible, indicating a large amount and some variation in size . In the RET channel, reticulocytosis is particularly striking.

Peripheral blood

In the smear, the reticulocytosis as noted in the RET channel is readily apparent; polychrome erythrocytes are plentiful. If you look closely, you can also see some erythrocytes with howell-Jolly bodies.

Thrombocytes

The platelet count is 1065*10^9/L, which is physiological for the mouse. In humans, such values are seen almost exclusively after splenectomy or in myeloproliferative disorders, such as Chronic Myeloid Leukemia, Essential Thrombocytosis or Primary Myelofibrosis. Additionally, the marked variation in shape and size of the platelets is striking.

Neutrophils

Neutrophils are relatively small, hypogranular and show slight hypersegmentation. Some neutrophils have an O-shaped nucleus; this morphology is considered the equivalent of the rod-core neutrophil in humans.

Lymphocytes

Mouse peripheral blood may consist largely (up to about 90%) of lymphocytes. The frequent occurrence of damaged cells is striking. Mouse lymphocytes are fragile and are easily smudged during bloodfilm preparation.

Monocytes

The monocytes exhibit a morphology largely similar to that in humans.

Eosinophils

Eosinophilic granulocytes are also morphologically very similar to those of humans, although the nuclear segmentation may be slightly less pronounced and more alike that of a monocyte.